| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
An alternative method for Staphylococcus aureus DNA isolation
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| Autores: |
Chapaval,L.
Moon,D.H.
Gomes,J.E.
Duarte,F.R.
Tsai,S.M.
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| Data: |
2008-04-01
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| Ano: |
2008
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| Palavras-chave: |
Staphylococcus aureus
Genomic DNA
DNA isolation
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| Resumo: |
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/S0102-09352008000200004
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.2 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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